Cellab® Bioreactor System

Insert Supply System - Disposable set

  • Easy to use disposable cell culture system
  • 2-compartment technology leads to high-density cell culturing
  • Automated cultivation process provides high level of reproducibility
  • Large media reservoir saves handling time and reduces consumables
  • Parallel cell cultivation in up to 24 inserts
Cellab Disposable Set Insert Supply System

The Insert Supply System represents a highly efficient bioreactor configuration suitable for small scale biomass or protein production as well as tissue cultivation like human artificial skin models.
The heart of the bioreactor is flat membrane inserts that are placed in a Media Supply Plate. Its large media reservoir has the same footprint as a standard multiwell plate. The semipermeable flat membrane in each insert is made from different materials and properties depending on the application. The membrane forms a two compartment system by separating cells from medium and allows high density cell culturing with improved culture quality.
The Insert Supply System is available either as a manual set or fully integrated in a Cellab® Disposable Set operated by the Cellab® Docking Station.

Features & Benefits

Semipermeable membrane

Cellab Products semipermeable membrane

The porous surface structure of the membrane provides an in-vivo like culture environment with excellent nutrient supply.

The membrane allows a free mass transfer via diffusion between the cell and the medium compartment. The defined membrane cut-off retains cells, proteins and high molecular weight growth supplements in the cell compartment.

The result is a high concentrated culture product and less need for growth factor supplementation.

2-compartment technology 

Cellab Products 2 compartment technology

The semipermeable membrane physically separates the bioreactor in a medium and a cell compartment. The membrane allows continuous diffusion of nutrients to the cell compartment on top of the membrane with a concurrent removal of metabolites.

This enables long-term cell culturing with high yields of concentrated cell culture products.

Parallel cultivation

Cellab Parallel Cultivation

The Insert Supply System provides a cell culture platform that allows for the parallel cultivation of up to 24 individual cultures. Each insert represents an individual growth chamber.

The scale up process can be extended beyond the insert volume by using hollow fiber bioreactors with increased surface to volume ratio.

Cellab Design SimplicityDesign simplicity of the Insert Supply System

The Insert Supply System combines a Media Supply Plate (MSP) and up to 6 flat membrane-based cell culture inserts. The MSP is one large media reservoir with the footprint of a standard multiwell plate. The membrane inserts are secured in the insert carrier plate that is fixed in the MSP. The insert carrier plate prevents floating of the inserts. The Insert Supply System enables cultivation of up to 6 different cell cultures in parallel, each contained in an individual insert.

It is possible to order the Insert Supply System already integrated in a Cellab® Disposable Set. This provides an integrated liquid flow path and connects the set to the peristaltic pump of the Cellab® Docking Station. It allows semi-automated media exchange without manual intervention and therefore reduce the risk of contamination. This is currently unique in the market. The increased media reservoir volume from the disposable set of up to 1.5 liter extends the media exchange cycles and reduces handling time.

Flexibility with different membrane types:

Regenerated celluloses

Cellab Cellulose membrane

  • Amorphous gel-like structure
  • High mechanical strength
  • Low protein binding
  • Excellent solvent resistance
  • Hydrophilic

Ideal suited for molecular filtration, separation and purification of proteins, antibodies, enzymes, viruses etc.

Polycarbonate

Cellab Polycarbonate membrane

  • Uniform porous structure with narrow pore size distribution and high pore density
  • Low protein binding
  • Good support for adherent cell cultures
  • Hydrophilic

Ideal suited for micro filtration, separation, tissue engineering, biomass production

Plug & Play cell culture System

The Cellab® Bioreactor System can easily be integrated in your existing lab infrastructure and cell culturing techniques without any specific requirements for your laboratory, materials or media. The entire system is designed to fit in a standard CO2-incubator and the associated software runs on a standard Windows computer without the need for a permanent connection.

The disposable set is delivered fully assembled and pre-sterilized. Once the disposable set is connected to the docking station and the media is added, the cell culture process can be started.

Automation for reproducible cell culturing

Cells cultured in the flat membrane inserts are automatically maintained by a medium and gas pump resulting in less manual interventions than traditional T-Flask culturing requires. There is no need for cell passaging and manual media changes, which significantly reduces labor and consumables, as well as reduces the risk of contamination.

Applications

Bioreactor systems using flat membrane inserts form an ideal environment for adherent and suspended cells. These bioreactors protect against shear stress and mimic the way cells grow in the human body which is superior to static processing in cell culturing vessels like T-Flasks. It is applicable to either serum-supplemented or serum-free cell cultures.

This makes the disposable set with the Insert Supply System suitable for a broad range of different applications including:

Protein production

Lab- and small-scale protein production of up to 100 mg of highly concentrated cell products (e.g. hybridoma based monoclonal antibody production, recombinant protein production etc.)

Biomass production

Culturing up to 4x106 adherent or suspended cells (e.g. mesenchymal stem cells)

Tissue engineering

Culturing artificial tissues (e.g. production of human artificial skin models for toxicity or skin compatibility testing, skin replacements etc.)

Using up to 4 Media Supply Plates with up to 24 integrated inserts allows parallel cultivation with a single disposable set for various academic or commercial research applications. It facilitates conducting parallel experiments for evaluating novel cell lines or media formulations.

Cellab also allows for scalability of your production process that makes it suitable from the very beginning of your cell culture process development up to pilot scale production. As a result, it allows your cell culture process to scale from lab to market with greater pace and efficiency by minimizing costs and shortening timelines.

 Versatility in the biotech landscape

  • Tissue engineering
  • Toxicity testing
  • Drug discovery
  • Air liquid interface cultivation
  • Co-culture studies

Applications overview

Technical specification & ordering information

Disposable sets for the automated system operating with the Cellab® Docking station

Cellab Ordering Information Insert Supply System Automated

Manual Insert Supply System

Cellab Ordering Information ISS Manual
Cellab Manual Insert Supply System

All Cellab® Disposable Sets are intended for single use only and will be delivered sterilized by radiation. All our products are delivered fully assembled and ready to use with an initial set of sterile filters, caps and one medium transfer system for the media filling process.

Feel free to contact us for more information about our products and services.

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