Protein Production with the Cellab® Bioreactor System
With the Cellab® Bioreactor System you can achieve:
- High protein concentration with minimal intervention
- Long term cultivation with multiple harvests but without the need for passing
- Reduced media and consumables use
- Parallel cultivation and reduced handling time
- Lower overall cost per culture
Benefits for protein production
Proteins are essential in nature and take different conformations depending on their function. Today most therapeutic proteins are made using recombinant DNA technology and genetically modified cells.
The Cellab® Bioreactor System allows you to increase the efficiency of your protein production by using our semi-automated hollow fiber bioreactor system or our scaffold holder system.
These easy-to-use systems provide the optimal conditions for different kinds of cell cultivation.
Producing monoclonal antibodies from different hybridoma cell lines using Cellab® Diaserts (dialysis membrane based inserts) increases the concentration of antibodies production by about 20 times compared to antibodies produced with T-Flasks or roller bottles.
Similar results are shown for hollow fiber bioreactor systems where the semipermeable membrane enables cells to be cultured at a very high density.
Despite the small housing of the bioreactor, the semipermeable membrane provides a very high surface area. In addition, the continuous circulation of nutrient and oxygen-rich media along with continuous removal of metabolites and CO2 from the cell side allows for an optimal cultivation of the cells.
The Cellab® Bioreactor System helps you to reach high density cell concentration combined with high protein concentration.
While producing monoclonal antibodies from hybridoma cells using the Cellab® hollow fiber bioreactor, the results obtained by our customers have shown that after 44 days of cell culture and 3 harvests, a total of 56.5 mg of monoclonal antibodies were harvested from a total volume of 13.9 ml.
Multiple cell lines can be maintained in the hollow fiber bioreactors for up to 3 months while a continuous harvest of protein is possible. No bioreactor replacement or transference is necessary in that period.
The Cellab® Bioreactor System provides a higher antibody concentration over a long period of time due to the high cell density.
One of our customers compared Cellab® Hollow Fiber Bioreactor with a T-Flask and a conventional two-compartment bioreactor system (miniPERM®). The results show the highest monoclonal antibody yield with the lowest volume of supernatant in the Cellab® Bioreactor System.
With the Cellab® Bioreactor System, reduced media and purification consumables are needed to isolate and purify the harvested antibodies. The total volume that must be processed to obtain the purified antibodies decreased tremendously. This results in lower media, purification and labor costs as well as less handling time.
The Cellab® Bioreactor System allows for the cultivation of up to 24 different hybridoma cell lines while using dialysis membrane-based inserts (Diaserts), and up to 3 different hybridoma cell lines with hollow fiber bioreactors.
Parallel cultivation reduces handling time and consumables required.
The Cellab® Bioreactor System provides a high cell density production combined with high protein concentration while lowering the overall cost of production. It enables the production of proteins with less handling (as opposed to T-Flasks which require a significant amount of manipulation for cell splitting and medium change as well as a large volume of supernatant and space for the development of the culturing process). Reducing handling and volumes also reduces the risks of contamination.
With the Cellab® Bioreactor System, the total cost per culture is reduced by up to 60%, while at the same time offering superior control of the cell culturing and reducing purification and labor costs.
The Cellab® Bioreactor System at a glance:
- Easy-to-use and reliable disposable bioreactor system
- Cultivates high cell densities
- Minimal manual intervention
- Reduced media volume
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